Were validated using RQ-PCR in an independant cohort of 93 early stage
Were validated using RQ-PCR in an independant cohort of 93 early stage CLL patients for 17 genes with gene-specific primers (Additional file 1: Table S1). The experiments were performed using SYBR Green Master Mix according to the manufacturer’s protocol on Mx3005P (Agilent Technologies, Santa Clara, CA, USA). The fold change was calculated using 2-Ct method … Read more